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This work aims to identify 6 virus field isolates, serologically determined as reoviruses. The type of cytopathic effect in cell cultures, physico-chemical properties and antigenic relationship were examined. The strains examined multiplied in CEF and CEK cultures, produced cytopathic effects and plaques, contained RNA in their genome, had no lipid envelope, were stable in an acid environment and...
Studies on the effects of freezing meat with spiked hormones at residue levels have been made. Beef and poultry meat samples which contained diethylstilbestrol and 19-nortestosterone were stored frozen at -20°C. The hormones were quantified by high-performance liquid chromatography with UV detection after 2, 4 and 6 months. Diethylstilbestrol is stable under all of these conditions and shows only...
This work aimed to establish correlation between concentration of zinc, copper and calcium in seminal plasma and characteristics of semen of stallions of various breeds (Thoroughbred -TB, Great Polish Horses -GPH, Polish Primitive Horses -PPH) and aged (≤ 11 years, > 11 years, > 14 years ). Measurements revealed interbreed differences in the concentration of zinc (TB:GPH, TB:PPH), copper (PPH:TB)...
In vitro effect of sub-inhibitory concentrations of penicillin and erythromycin on the number, growth rate, and cultural and biochemical characteristics of Staphylococcus aureus was examined. A decreased number and a slow growth of Staph.aureus in broth and minced meat comprising selected concentrations of antibiotics, and alterations in enzymatic and biochemical activities were found.
The kinetics of radioruthenium in rats after repeated contamination with small doses was investigated. Ruthenium-106 was poorly absorbed and rapidly eliminated from the organs and tissues of rats. No specific affinity of Ru-106 for any organ or tissue was observed except for the kidneys, where marked tendency for cumulating was found. It seems that radioruthenium is of less importance for the human...
The aim of presented preliminary studies was the application of PCR for rapid and accurate diagnosis of CAV-1 infection in dogs. The 301 bp PCR product of CAV-1 was demonstrated in two lung and liver homogenates collected post-mortem from two dogs. The comparable studies of cytopathic effect evaluated at the 10th passage of homogenates in MDCK cells and PCR revealed 100% accordance of the results...
The course of S. papillosus infection in lambs born by ewes affected with this disease was estimated on the basis of the presence of parasitic eggs in faeces. The examinations were started 2 weeks prior to the expected parturition and were continued in lambs and their mothers at 2-week intervals until the lambs were 100 days old. It was demonstrated that 60% or the lambs had been infected with S....
Standard Pb, Cd, Zn and Cu additives were investigated for their effects on the fermenting activity of Lactobacillus helveticus and Lactobacillus casei. In milk enriched with a Lb. helveticus dairy culture and containing the standard Cu additive, activation of dairy bacteria could be observed. Standard amounts of Pb and Zn did not cause changes in titration acidity when compared to the control sample...
Experiments were performed on 12 sheep divided into 2 groups and fed a standard diet. The trial group received the same nutritive fodder supplemented with 0.3 ppm of sodium selenite (Na₂SeO3). The concentrations of selenium, triiodothyronine and thyroxine in serum were determined every 4 weeks during 20 weeks, respectively. Oral supplements of sodium selenite increased the serum concentration of selenium...
Levels of nitrates and nitrites were observed in pooled samples of milk from selected suppliers of the Košice dairy, and in dairy treated milk during certain months of the year. The average value of nitrates in examined (432) pooled samples of milk ranged from 1.84 to 4.58 mg of NaNO₃.1⁻¹, and in dairy-treated milk (72 samples), from 2.33 to 6.23 mg of NaNO₃.1⁻¹. In the both cases maximal values of...
The studies aimed to induce endotoxin tolerance in horses and to evaluate this state with regard to clinical observations. Investigations involved 8 clinically healthy mares subjected to 4 iv. injections with 0.1 μg/kg b.w. doses of LPS from E. coli each; the three fast doses (LPS-1, LPS-2, and LPS-3) were given daily whereas the fourth injection (LPS-4) was performed one week after LPS-3. Rectal...
These investigations aimed to evaluate the influence of acaricides used in Bulgaria (based on amitraz, cumaphos and fluvalinate) on some factors of the non-specific bee immunity in the appearance of chalkbrood. It was found that the changes of protein concentration and lysozyme amount in the haemolymph and the pH of bee intestine depend on different active substances of the drugs. Field trials canied...
The reverse transcriptase-polymerase chain reaction (RT-PCR) was used to amplify the first part or full capsid gene (VP60) of EBHSV and RHDV. The fragment covered full capsid gene and the most part of gene contained in ORF2 of EBHSV was also obtained. The specificity of PCR products was checked by digestion with restriction enzymes.
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